Coding

Part:BBa_K2581014

Designed by: Laura Sans Comerma   Group: iGEM18_UPF_CRG_Barcelona   (2018-10-09)


FadE gene

The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the DH5-alpha E.coli strain. It consists of the coding sequence of the acyl-coA dehydrogenase (FadE) gene which converts acyl-coA into enoyl-coA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 584
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 367
    Illegal SapI.rc site found at 1240
    Illegal SapI.rc site found at 2383


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Categories
Parameters
None